Composite

Part:BBa_K594014

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-10-01)


A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.

This composite is a device of function. it is composed of ptetR(R0040), RBS(B0034), cinR(C0077), double terminator(B0015), pcin(R0078), RBS(B0030), luxI(C0061), RBS(B0032), GFP(E0030), double terminator(B0015).

When IPTG is added, the plac is activated, producing luxR, and then the plux will be activated when 3-O-C6-HSL is added. The 3-O-C6-HSL units with luxR and form a complex that can bind to the lux cassette in plux so that the transcription of lasI, leuB and ECFP happen, producing 3-O-C12-HSL,leuB and the ECFP reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 608
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2577


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Categories
Parameters
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